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何中良 Chung-Liang Ho

Chung-Liang,Ho

何中良  病理學科 教授 (Chung-Liang Ho)

E-mail:clh9@mail.ncku.edu.tw

電話:06-2353535 分機2638

傳真

 

學經歷

 

學歷
1997

美國哥倫比亞大學博士

 
1990

國立台灣大學醫學系醫學士

   

 

現職 2006- 國立成功大學附設醫院病理部分子病理科 科主任
  2005- 國立成功大學醫學系病理學科 副教授
  2001- 國立成功大學附設醫院病理部 主治醫師
 
 

經歷

2003-2004 美國約翰霍普金斯醫學中心婦科病理研究員

 

2001- 國立成功大學附設醫院病理部 主治醫師

 

2001-2005 國立成功大學醫學系病理學科 助理教授 (分子醫學研究所合聘)

 

1999-2001 國立成功大學附設醫院病理部 總住院醫師

 

1997-1999 國立成功大學附設醫院病理部 住院醫師

 

1990-1991 台北醫學病理科 助教兼住院醫師

 

專長/研究興趣

 

病理學、癌症生物學、生物資訊

   

 

興趣:

我的實驗室主要在整合細胞生物學、生物資訊學、以及分子病理學等方法,來從事腫瘤誌(Tumor marker)之開發與研究。觀諸現有的腫瘤標誌,以下列兩類在正常細胞中較不會出現:

1.腫瘤胚基因/蛋白(Oncofetal genes/proteins):以甲種胎兒蛋白(Alpha fetal protein; AFP)為代表。這類基因在正常成人細胞中表現量極低或完全不表現。

2融合基因(Fusion genes):以白血病常見的bcr/abl、pml/rara等為代表。此類融合基因在正常細胞代表 不會出現。

我們運用自己開發的生物資訊工具來尋找新的腫瘤胚基因,最後找到了一群與細胞分化、胚胎發育極為相關,而在腫瘤又有表現的新穎基因,目前正積極進行研究,一方面運用病理檢體以及臨床資料,期能找出這些基因的臨床應用,另一方面則使用分子生物及細胞生物的方法,希望對這些基因的功能有進一步的了解。

在基因的功能方面,我們找到了一個新穎基因LRRC16B,具備leucine rich region domain,由XTTsoft agar以及Xenograft等研究顯示該基因可以促進細胞proliferationtransformation。該新穎基因的上下游、調控方式、cell cycle的影響、在胚胎發育及腫瘤生成所扮演的角色等等,尙有許多待值得深入探討的地方。除此之外,尚有3個新穎基因經chromatin immunoprecipitation顯示為WntTarget genes,並且在肝癌組織中會過量表現,這些基因也值得進一步探討。

在基因的臨床應用方面,我們的生物資訊分析找到了Lin28B,進一步實驗顯示如果在肝癌細 病人的血流細胞中偵測到Lin28B的transcripts,則有明顯的早期復發的狀況。Lin28B與cancer stem cell之間的關係,也是一個有趣的課題。

除上述基因之外,尚有若干新穎基因初步看來頗為有趣,將來也會是值得探討的對象。 在融合基因方面,本實驗室分別開發出高通量以及高涵蓋率的方法來偵測融合基因。期望未來能夠發現新穎的融合基因,成為癌症診斷與治療的新標的。

 

 

榮譽獎項

 

 
 

 研究成果 (Publications) -- Chung-Liang Ho

 
  1. Chien-Hsuan Tai, Chung-Liang Ho*, Ya-Lan Chen, Wan Li Chen, Gwo-Bin Lee A novel integrated microfluidic platform to perform fluorescence in situ hybridization for chromosomal analysis Microfluidics and Nanofluidics, December 2013, Volume 15, Issue 6, pp 745-752

  2. Cheng SW, Tsai HW, Lin YJ, Cheng PN, Chang YC, Yen CJ, Huang HP, Chuang YP, Chang TT, Lee CT, Chao A, Chou CY, Chan SH, Chow NH, Ho CL. Lin28B is an oncofetal circulating cancer stem cell-like marker associated with recurrence of hepatocellular carcinoma. PLoS One. 2013 Nov 14;8(11):e80053.

  3. Yi-Lin Chen, Nan-Haw Chow, Cheng-Chan Lu, Wen-Ya Huang, Chung-Liang Ho*. The Design of Molecular Diagnostic Clinical Laboratory.生檢雜誌 2013;25:45-55.

  4. Chen YT, Chong YM, Cheng CW, Ho CL*, Tsai HW, Kasten FH, Chen YL, Chang CF. Identification of novel tumor markers for oral squamous cell carcinoma using glycoproteomic analysis.Clin Chim Acta. 2013 May;420:45-53.

  5. Chen YT, Ho CL, Chen PK, Chen YL, Chang CF. Anterior gradient 2: a novel sensitive tumor marker for metastatic oral cancer. Cancer Lett. 2013 Oct 10;339(2):270-8.

  6. Chung WP, Song HL, Ho CL*, Chiu NT, Su WC. Gastric perforation secondary to regression of lung adenocarcinoma after gefitinib treatment .J Clin Oncol.2013 Jan 1;31(1):e6-8.

  7. Yu CH, Yang SC, Lai WW, Liao YC, Ho CL, Su WC, Chang HY. Germ cell tumor with somatic-type malignancy presenting as right facial swelling in a young woman. J Clin Oncol. 2011 Aug 20;29(24):e699-701.

  8. Hsu CC, Chiang CW, Cheng HC, Chang WT, Chou CY, Tsai HW, Lee CT, Wu ZH, Lee TY, Chao A, Chow NH, *Ho CL(corresponding). Identifying LRRC16B as an oncofetal gene with transforming enhancing capability using a combined bioinformatics and experimental approach. Oncogene. 2011 Feb 10;30(6):654-67.

  9. Yeh YM, Chang KC, Chen YP, Kao LY, Tsai HP, Ho CL, Wang JR, Jones D, Chen TY.  Large B cell lymphoma presenting initially in bone marrow, liver and spleen: an aggressive entity associated frequently with haemophagocytic syndrome. Histopathology. 2010 Dec;57(6):785-95

  10. Lien KY, Chuang YH, Hung LY, Hsu KF, Lai WW, Ho CL, Chou CY, *Lee GB. Rapid isolation and detection of cancer cells by utilizing integrated microfluidic systems. Lab Chip. 2010 Nov 7;10(21):2875-86.

  11. Yeh YM, Chen YL, Cheng HY, Su WC, Chow NH, *Chen TY, *Ho CL (co-corresponding). High percentage of JAK2 exon 12 mutation in Asian patients with polycythemia vera. Am J Clin Pathol. 2010 Aug; 134(2) 266-70.

  12. Liu HS, Hsu PY, Lai MD, Chang HY, Ho CL, Cheng HL, Chen HT, Lin YJ, Wu TJ, Tzai TS, Chow NH. An unusual function of RON receptor tyrosine kinase as a transcriptional regulator in cooperation with EGFR in human cancer cells. Carcinogenesis. 2010 Aug;31(8):1456-64.

  13. Chen YL, Su IJ, Cheng HY, Chang KC, Lu CC, Chow NH, *Ho CL (co-corresponding), *Huang W. BIOMED-2 Protocols to Detect Clonal Immunoglobulin and T Cell Receptor Gene Rearrangements in B- and T-cell Lymphomas in Southern Taiwan. Leuk Lymphoma. 2010 Apr;51(4):650-5.

  14. *Sun YN, Lin CH, Kuo CC, Ho CL, Lin CJ. Live cell tracking based on cellular state recognition from microscopic images. JMicrosc. 2009 Jul;235(1):94-105.

  15. Cheng YM, Ho CL, Chiu NT, *Hsu KF. Cesarean section scar mimicking uterine malignant neoplasm at positron emission tomography/computed tomography. J Minim Invasive Gynecol. 2009 May-Jun;16(3):372-4.

  16. Yen KH, *Ho CL (corresponding), Lee C.  The analysis of inconsistencies between cytogenetic annotations and sequence mapping by defining the imprecision zones of cytogenetic banding. Bioinformatics. 2009 Apr 1;25(7):845-52.

  17. Chen JC, Ho CL, Tsai HW, Tzai TS, Liu HS, Chow NH, Yang WH, *Cheng HL. Immunohistochemical detection of prostate-specific antigen expression in primary urothelial carcinoma of the urinary bladder. Anticancer Res. 2008 Nov-Dec;28(6B):4149-54.

  18. Hsing CH, Li HH, Hsu YH, Ho CL, Chuang SS, Lan KM, *Chang MS.  The distribution of interleukin-19 in healthy and neoplastic tissue. Cytokine. 2008 Nov;44(2):221-8.

  19. Tsai HW, Lin CP, Chou CY, Li CF, Chow NH, Shih IM, *Ho CL (corresponding). Placental site nodule transformed into a malignant epithelioid trophoblastic tumour with pelvic lymph node and lung metastasis. Histopathology. 2008Nov;53(5):601-4.

  20. Ho CL, Tzai TS, Chen JC, Tsai HW, Cheng HL, Eisenberger CF, *Chow NH. The molecular signature for urothelial carcinoma of the upper urinary tract. J. Urol. 2008 Mar;179(3):1155-9.

  21. Chang YS, Huang FC, Tseng SH, Hsu CK, Ho CL, *Sheu HM. Erythema multiforme, Stevens-Johnson syndrome, and toxic epidermal necrolysis: acute ocular manifestations, causes, and management. Cornea. 2007 Feb;26(2):123-9.

  22. Hsu PY, Liu HS, Cheng HL, Tzai TS, Guo HR, Ho CL, *Chow NH. Collaboration of RON and Epidermal Growth Factor Receptor in Human Bladder Carcinogenesis. J Urol. 2006 Nov;176(5):2262-7.

  23. Hsing CH, Ho CL, Chang LY, Lee YL, Chuang SS, *Chang MS. Tissue microarray analysis of interleukin-20 expression. Cytokine. 2006 Jul;35(1-2):44-52.

  24. Lai TH, Zhao Y, Shih IeM, Ho CL, Bankowski B, *Vlahos N. Expression of L-selectin ligands in human endometrium during the implantation window after controlled ovarian stimulation for oocyte donation. Fertil Steril. 2006 Mar;85(3):761-3.

  25. Lin CP, Ho CL, Shen MR, Huang LH, *Chou CY. Evidence of human papillomavirus infection, enhanced phosphorylation of retinoblastoma protein, and decreased apoptosis in sarcomatoid squamous cell carcinoma of uterine cervix. Int J Gynecol Cancer. 2006 Jan-Feb;16(1r):336-40.

  26. Chen WC, Jones D, Ho CL, Cheng CN, Tseng JY, Tsai HP, *Chang KC. Cytogenetic anomalies in hyaline vascular Castleman disease: report of two cases with reappraisal of histogenesis. Cancer Genet Cytogenet. 2006 Jan 15;164(2):110-7.

  27. Tsai HW, Chow NH, Lin CP, Chan SH, Chou CY, *Ho CL (corresponding). The significance of prohibitin and c-Met/HGFR expressions in the progression of cervical adenocarcinoma. Human Pathology. 2006 Feb; 37(2):198-204.

  28. Lo CA, Huang SC, Ho CL, Tzeng CC, Chou CY, *Hsu KF.  Discordant responses to progestin in a patient with uterine low-grade smooth-muscle tumors metastatic to the lung. Journal of Obstetrics and Gynaecology research. 2005 Oct; 31(5):394-8.

  29. Yen KH, Lee C, Liu HS, *Ho CL (corresponding). A precise and scalable method for querying genes in chromosomal banding regions based on cytogenetic annotations. Bioinformatics. 2005 Sep; 21(17):3469-74.

  30. Chien CL, Liu TC, Ho CL, *Lu KS. Overexpression of neuronal intermediate filament protein alpha-internexin in PC12 cells. Journal of Neuroscience Research 2005 Jun; 80(5):693-706.

  31. Cheng HL, Liu HS, Lin YJ, Chen HH, Hsu PY, Chang TY, Ho CL, Tzai TS, *Chow NH. Co-expression of RON and MET is a prognostic indicator for patients with transitional-cell carcinoma of the bladder. British Journal of Cancer. 2005 May 23; 92(10):1906-14.

  32. Lai TH, Shih IeM, Vlahos N, Ho CL, Wallach E, *Zhao Y. Differential expression of L-selectin ligand in the endometrium during the menstrual cycle. Fertility and Sterility. 2005 Apr; 83 Suppl 1:1297-302.

  33. Pohl G, Ho CL (equal contribution), Kurman RJ, Bristow R, Wang TL, *Shih IeM. Inactivation of the mitogen-activated protein kinase pathway as a potential target-based therapy in ovarian serous tumors with KRAS or BRAF mutations. Cancer Research. 2005 Mar; 65(5):1994-2000.

  34. Ho CL, Mou TY, Chiang PS, Weng CL, *Chow NH. Mini chamber system for long-term maintenance and observation of cultured cells. Biotechniques. 2005 Feb; 38(2):267-73.

  35. Dai YC, Ho CL (equal contribution), Tsai YC, Hsu YH, Chen HW, Liu HS, Chang YC, *Chow NH. Allelic loss of 14q32 in the pathogenesis of gastrointestinal and ampullary malignancies. Mapping of the target region to a 17 cM interval. Journal ofCancer Research and Clinical Oncology. 2005 Feb; 131(2):94-100.

  36. Su SJ, Yeh TM, Chuang WJ, Ho CL, Chang KL, Cheng HL, Liu HS, Cheng HL, Hsu PY, *Chow NH. The novel targets for anti-angiogenesis of genistein on human cancer cells. Biochemical Pharmacology. 2005 Jan; 69(2):307-18.

  37. Chang YS, Ho CL, *Tseng SH. Socket infection due to retained gauze after evisceration. Ophthalmic Surgery Lasers & Imaging. 2004 Nov-Dec; 35(6):503-6.

  38. Ho CL, Kurman RJ, Dehari R, Wang TL, *Shih IeM. Mutations of BRAF and KRAS precede the development of ovarian serous borderline tumors. Cancer Research2004 Oct; 64(19):6915-8.

  39. Hsu CY, Bristow R, Cha MS, Wang BG, Ho CL, Kurman RJ, Wang TL, *Shih IeM. Characterization of active mitogen-activated protein kinase in ovarian serous carcinomas. Clinical Cancer Research. 2004 Oct; 10(19):6432-6.

  40. Huang SC, Tang MJ, Cheng YM, Hsu KF, Ho CL, *Chou CY. Enhanced polyadenosine diphosphate-ribosylation in gonadotropin-releasing hormone agonist-treated uterine leiomyoma. Journal of Clinical Endocrinology and Metabolism.2003. Oct; 88(10):5009-16.

  41. Tzai TS, Chen HH, Chan SH, Ho CL, Tsai YS, Cheng HL, Dai YC, Lin JS, Yang WH, *Chow NH. Clinical significance of allelotype profiling for urothelial carcinoma. Urology. 2003. Aug; 62(2):378-84.

  42. Hsu YJ, Pai L, Chen YC, Ho CL, Kao WY, Chao TY.  Extragonadal germ cell tumors in Taiwan: an analysis of treatment results of 59 patients. Cancer. 2002 Aug 15;95(4):766-74.

  43. Chang YC, Ho CL, Chen HH, Chang TT, Lai WW, Dai YC, Lee WY, Chow NH.  Molecular diagnosis of primary liver cancer by microsatellite DNA analysis in the serum. British Journal of Cancer. 2002. 87(12):1449-53.

  44. Cheng HL, Trink B, Tzai TS, Liu HS, Chan SH, Ho CL, Sidransky D, Chow NH.  Overexpression of c-met as a prognostic indicator for transitional cell carcinoma of the urinary bladder: a comparison with p53 nuclear accumulation.  Journal ofClinical Oncology. 2002.  20(6):1544-50.

  45. Chow NH, Chan SH, Tzai TS, Ho CL, Liu HS.  Expression profiles of erbb family receptors and prognosis in primary transitional cell carcinoma of the urinary bladder. Clinical Cancer Research. 2001. Jul;7(7):1957-62.

  46. Cheng HL, Tong YC, Tzai TS, Weng CL, Ho CL, Yang WH, Lin JS, Chow NH.  Expression of nm23-H1 in transitional cell carcinoma of the upper urinary tract. Oncology Reports. 2001. Jan-Feb;8(1):193-6.

  47. Wang L, C.-L. Ho, D. Sun, R.K. Liem, A. Brown.  Rapid movement of axonal neurofilaments interrupted by prolonged pauses.Nature Cell Biology. 2000. Mar; 2(3):137-41.

  48. Martys, J.L., C.-L. Ho, R.K.H. Liem and G.G. Gundersen. Intermediate filaments in motion: observations of intermediate filaments in cells using green fluorescent protein-vimentin. Molecular Biology of the Cell. 1999. 10:1289-95.

  49. Ho, C.-L., J.L. Martys, A. Mikhailov, G.G. Gundersen and R.K.H. Liem.  Novel features of intermediate filament dynamics revealed by green fluorescent protein chimeras. Journal of Cell Science. 1998. 111: 1767-1778.

  50. Ho, C.-L., C.-L. Chien, K. Brown and R.K.H. Liem. Ultrastructural studies of carboxyl-terminal truncation mutants of the neuronal intermediate filament protein, peripherin. Journal of Neurochemistry. 1998. 70:1916-1924.

  51. Angelastro, J.M., C.-L. Ho, T. Frappier, R.K.H. Liem and L.A. Greene. Peripherin is tyrosine-phosphorylated at its carboxyl-terminal tyrosine. Journal of Neurochemistry. 1998. 70:541-549.

研討會論文
  1. Yi-Lin Chen (陳怡伶), Hsieh-Yin Cheng (鄭雪吟), Cheng-Chan Lu (呂政展), Wen-Ya Huang(黃溫雅), Chung-Liang Ho (何中良)*  The initial experience of CAP proficiency test for molecular oncology at National Cheng Kung University Hospital. (2009, May, 台北市, 2009台灣病理學會暨國際病理學會台灣分會年會)

  2. Wen-Ya Huang(黃溫雅)*, Yi-Lin Chen(陳怡伶), Hsieh-Yin Cheng(鄭雪吟), Cheng-Chan Lu(呂政展)Chung-LiangHo(何中良)* . A Comparison between Nested PCR and BIOMED-2 Protocols for the Detection of Clonal Immunoglobulin Heavy Chain and T Cell Receptor Gene Rearrangement.  (2007, May, 台北市, 2007台灣病理學會暨國際病理學會台灣分會年會)

  3. 何中良 Cytoband Query System (CQS) and Its Application in the Systematic Analysis of Positional Conflict Between Cytogenetic and Sequence Mapping Information (2006, May, 台北市, 2006台灣病理學會暨國際病理學會台灣分會年會)

  4. 徐中濟何中良.  Chung-Chi Hsu, and Chung-Liang Ho. New identification of bio-markers with oncofetal-like featurers.  (2006, January; 墾丁第十五屆細胞及分子生物新知研討會)

  5. C-L HoRJ Kurman, T-L Wang, I-M Shih. Mutations of BRAF and KRAS Precede the Development of Ovarian Serous Borderline Tumors. (2005, February; San Antonio; USCAP annual meeting)

  6. C-L HoG Pohl, RJ Kurman, T-L Wang, I-M Shih. The Roles of Mitogen-Activated Protein Kinase in Serous Borderline Tumor and Low-Grade Serous Carcinoma. (2005, February; San Antonio; USCAP annual meeting)

  7. 嚴國何黃中偉何中良.  Kuo-Ho Yen, Chung-Wei Huang, and Chung-Liang Ho.  A versatile algorithm for cytoband query and its integration with EST expression profiling tools.  (2003, January; 墾丁第十一屆細胞及分子生物新知研討會)

  8. 江旆萱牟敦毅何中良.  Pei-Shuan Chiang, Tun-Yi Mon, and Chung-Liang Ho.  Wound healing motility assay revisited- aided by a novel long-term, time-lapse recording system.  (2002, November; 台北市; APOCB)

  9. 翁茱莉牟敦毅何中良.  Chu-Li Weng, Tun-Yi Mon, and Chung-Liang Ho.  Unexpected “Gliding” Movements of the Cell Bodies of a Neuronal Cell Line on its Neurites Revealed by Long-Term Time-Lapse Recording.  (2001, February; 墾丁第九屆細胞及分子生物新知研討會)

專利
  1. 名稱:顯微鏡台細胞培養皿
    申請案號:
    090222311
    申請日期:
    2001/12/19
    創作人:何中良、牟敦毅
    申請人:國立成功大學
    公告編號:
    581119
    公告日期:
    2004/03/21
    專利核准日期:
    2004/07/19
    專利號碼:新型第
     221388 
    專利權期間:
    2004/03/21 ~ 2013/12/18
     

  2. 國外專利
    Patent No.: US 6,727,089 B2
    Date of Patent: Apr. 27, 2004
    Duration: 20 years
    Culturing chamber on microscope stage
    Inventors: Chung-Liang Ho; Tun-Yi Mou
    Assignee: National Cheng Kung University.
    Appl. No.: 10/320,552
    Filed: Dec. 17, 2002

 

 
實驗室
 
  研究生: 徐中濟、陳疇丞、鄭舒文、陳怡文、陳菀莉
研究助理: 陳雅蘭

 

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