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Laboratory  

 
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Curriculum Vitae
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Publications
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Other

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Shainn-Wei Wang, Ph.D.

E-mailGswwang@mail.ncku.edu.tw 

TELG06-2353535 ext 4218(off.) ext 4217(lab.)

FAXG06-2095845

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Educations / Professional Experience

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Educations

Diploma:

  • 9/1996-9/1992: Ph.D. The Pennsylvania State University (Genetics)

  • 9/1992-9/1990: M.S. The Pennsylvania State University (Genetics)

  • 1983-1987:  B.S. Fu-Jen Catholic University (Biology)

@ Thesis:
  • Wang, S.-W. (1996). Biochemistry and cellular biology of the interaction of Bacillus thuringiensis CryIC toxin with Spodoptera exigua midgut epithelial cells in vitro.  The Pennsylvania State University.  Ph.D. dissertation.

  • Wang, S.-W. (1992). Purification and characterization of the Platinota idaeusalis baculovirus pathogenic to Apple bud moth. The Pennsylvania State University.  M.S. dissertation

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Current Position 8/2005-current Adjunct Assistant Professor,The National Health Research Institute of Clinical Medicine and Department of Pathology
@ 8/2004-current Adjunct Assistant Professor,The Institute of Basic Medical Science of NCKU
@ 8/2004- current

Assistant Professor,Institute of Molecular Medicine, National Cheng Kung University Medical College

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Professional Appointments

@ 9/1997- 7/2004 Postdoctoral Research Fellow, Harvard Medical School/Childrens Hospital
@ 9/1996-9/1997 Postdoctoral Research associate, Pesticide Research Laboratory, Inter-college program in Genetics, The Pennsylvania State University
@ 1993-1996 Research assistant, Pesticide Research Laboratory, The Pennsylvania State University
@ 1989-1990 Research technician, Fu-Jen Catholic University, Microbiology Laboratory
@ 1986-1987 Internship (HPLC certificate), Asian Vegetable Research and Development Center, Plant physiology Department
@ 1985-1986 Internship: Fu-Jen Catholic University, Plant Ecology La
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@ Major Administration Activities & Services
  • 8/2004-8/2009: Bio-safety P3 Lab Committee, NCKU, College of Medicine

  • 8/2004-8/2009: IMM Faculty Recruitment Committee, NCKU, Medical School

  • 8/2005-8/2009: IMM Graduate Student Recruitment Committee

  • 8/2005-8/2006: IMM Teaching Coordination and Improvement Committee, NCJU, Medical School

@  Affiliations
  • AAAS (American Association for the Advancement of Science)

  • SAPA (Sino-American Pharmaceutical Professionals Association-New England)

  • GSAS Harvard Biotechnology Club

  • TSAB (Taiwan Scholar Association in Boston)

  • TAS (Taiwan AIDS Society)

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Expertise /Research Interests

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Immunology/DNA vaccine; mucosal and cellular immunity

Advanced Virology/Biology of RNA viruses; viral-host interaction and pathogenesis

Cellular Biochemistry/Protein biochemistry; vesicular trafficking; membrane signaling

Molecular Genetics/Transcriptional and translational regulations

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Current research focus:

The coordination between viral proteins and host proteins are required to support a virus life cycle, which may interrupt many intricate cellular activities to favor viral gene replication and the formation of infectious viral entities. What kinds of cellular proteins are hijacked by viral proteins for viral entry, replication, assembly and budding? Are there any antiviral molecule, restriction factor, or sensor in the host cells to be antagonized during viral infection for immune or non-immune escape? Can we dissect such intricate virus Vhost interactions into subcellular pathways and molecular levels to advance our knowledge for the development of better antiviral drugs, vaccine, and diagnosis tools? These are the basic questions and scientific pursues of my laboratory. My current research directions mainly involve the understanding of host factors participates in Hepatitis C virus (HCV) and Enterovirus (EV-A71) infection and pathogenesis. Ongoing projects include: (1) Identification of critical cellular factors or subcellular interactome(s) of HCV core protein in liver and immune cells for viral infection and pathogenesis; (2) Identification of critical cellular factors or subcellular interactome(s) of EV-A71 structural proteins for viral infection and pathogenesis. (3) Use of Loop-mediated Isothermal Amplification (LAMP) or RT-LAMP for viral detection tools development. (4) Development of viral polymerase-specific DNA aptamers as antiviral molecules or detection reagents.

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  My long term goals are to:

(1) understand the pathogenesis of epidemic virus in Asia: We are interested in finding out how the gene networks are reprogrammed in DC, Macrophage, and T cells in responding to viral infections. Gene chips and Flow cytometry techniques will be used to investigate possible pathogenic mechanisms related to apoptosis or other cellular signaling events.

(2) identify host restriction factors for viral infection: For example, by comparing protein profiles of susceptible cells to those of non-susceptible cells when exposed to virus or viral proteins, we will investigate the cellular factor(s) that have direct protein-protein interaction with viral protein(s) in restricting viral entry, assembly, genome encapsidation, or replication. Tandem affinity purification for protein complex (Tap-Tag) and mass spectrometry will be used to explore the protein network related to the species-specific resistance of viral infection.

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  1. HIV vaccine development and mucosal immunity

  2. Identification of the cellular protein network for HIV nucleocapsid during Gag transport and selective RNA packaging

  3. Proteomic analysis of the HCV core-interacting cellular protein network in hepatocytes and immune cells

  4. Development of a multivalent retroviral/flaviviral chimeric vector vaccine against HCV and HIV.

  5. Investigation of Influenza A virus NS1 protein on the role of circumventing cytokine responses and the associated functional cellular protein network leading to increased virulence

  6. Effective detection of human immunodeficiency virus type 1 (HIV-1) in infants from eropositive pregnants by using padlock probes

  7. HIV surveillance & Resistance in Southern-Taiwan

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  1. understand the pathogenesis of epidemic virus in Asia: We are interested in finding out how the gene networks are reprogrammed in DC, Macrophage, and T cells in responding to viral infections. Gene chips and Flow cytometry techniques will be used to investigate possible pathogenic mechanisms related to apoptosis or other cellular signaling events.

  2. identify host restriction factors for viral infection: For example, by comparing protein profiles of susceptible cells to those of non-susceptible cells when exposed to virus or viral proteins, we will investigate the cellular factor(s) that have direct protein-protein interaction with viral protein(s) in restricting viral entry, assembly, genome encapsidation, or replication. Tandem affinity purification for protein complex (Tap-Tag) and mass spectrometry will be used to explore the protein network related to the species-specific resistance of viral infection